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KMID : 0364820140500040384
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Korean Journal of Microbiology
2014 Volume.50 No. 4 p.384 ~ p.386
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Isolation and Identification of Pseudomonas aeruginosa in Natural Environments by International Organization for Standardization ISO/NP 16266
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Lee Si-Won
Kim Ji-Hae
Lee Bo-Ram
Joo Youn-Lee
Choi Byeol
Park Su-Jeong
Chung Hyen-Mi
Jheong Won-Hwa
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Abstract
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Pseudomonas aeruginosa is an opportunistic pathogen that inhabits various natural and artificial environments, such
as pathogenesis, water, soil and air. They can cause serious problems, such as pathogenic infection. In this study, 220
colonies were isolated from water and soil environment that assumed to be P. aeruginosa using a membrane filter
method based on International Organization for Standardization (ISO/NP 16266). Identification of the isolates was
determined by physiobiochemical characteristics using newly modified ISO method which includes the resistance to
1,10 phenanthroline test. Only one of 220 presumed P. aeruginosa strains isolated from effluence water using a
drain swab was determined as P. aeruginosa-positive by the ISO/NP 16266 method. Subsequently, the resistance to
1,10 phenanthroline test, which was newly proposed by ISO in 2014 and applied in this study, was considered as
more precise and improvable method for identification of P. aeruginosa.
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KEYWORD
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Pseudomonas aeruginosa, International Organization for Standardization, ISO/NP 16266
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